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KMID : 0379120140420040282
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Korean Journal of Mycology
2014 Volume.42 No. 4 p.282 ~ p.288
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Identification and Characterization of Aspergillus oryzae Isolated from Soybean Products in Sunchang County
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Lim Eun-Mi
Lee Ji-Young
Mohammed A. Abdo Elgabbar
Han Kap-Hoon
Lee Bo-Soon
Cho Yong-Sik
Kim Hyoun-Young
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Abstract
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In this study, we attempted to isolate fungi from soybean fermented foods produced in Sunchang County and to identify Aspergillus oryzae from fungal isolates. Ten fungal isolates were identified with β-tubulin gene. According to the sequences of β-tubulin gene, ten fungal isolates were identified as A. oryzae/flavus complex. For further identification of the ten of fungal isolates, omtA gene, one gene of the aflatoxin biosynthesis gene cluster, was sequenced and the sequences were compared with those of A. oryzae and A. flavus strains from the GenBank database. In addition, identification of the ten fungal isolates was further confirmed using the PCR amplicon of norB and cypA intergenic region, in which a deletion was recognized relative to A. flavus and A. parasiticus. The amplicon size of the ten fungal isolate strains was smaller than those of A. flavus and A. parasiticus, but the same as that of the reference A. oryzae strain. These results indicated that the ten isolates should be identified as A. oryzae. The protease activity in rice koji made with 6, 13, 17, 27, 37 and 38 of strain, respectively was twice higher than that in control. The kojis made with nine of the A. oryzae isolates, respectively, did not produce aflatoxin, suggesting that the strains could possibly be used as starters for soybean products.
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KEYWORD
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Aspergillus oryzae, β-tubulin, Identification, Isolation, Mycotoxin
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